It is well known that bacteria and fungi hide various metabolites related to their pathogenesis. However, the effect of these metabolites on microbial virulence is poorly understood.
One well-known example of a metabolite is a quorum sensing molecule that allows microbes to share information about cell density and adjust gene expression. It allows organisms to modulate pathogenic processes, thus increasing virulence. Therefore, understanding the metabolome associated with the microbiome is an integral part of providing a complete picture of the microbial landscape.
NTS can assess the metabolome associated with microbiome analysis (tissue, stool, or culture models) and determine the identification of bacterial or fungal metabolites. Samples can be extracted in solution with internal standards and lyophilized. The technological approach allows for identification of metabolites, which includes amino acids, nucleotides, fatty acids, etc. and can be quantitatively observed using multiple reaction monitoring. Appropriate statistical measures are then applied to the data to identify metabolites that are significantly altered in each comparison.
NTS has an established relationship with the Department of Pharmacology at Case Western Reserve University and Dr. Masaru Miyagi.
NTS has an established relationship with the Department of Pharmacology at Case Western Reserve University and Dr. Masaru Miyagi, a dedicated expert in metabolomics and proteomics. The relationship enables the company to dive deeper into microbial metabolomics. Dr. Miyagi is an expert in mass spectrometry and application of mass spectrometry-based methodologies for proteome and metabolome analyses.
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