NTS partners with the Center for Medical Mycology to offer in vitro models of Candida biofilms to characterize formation on clinically relevant medical devices, such as dentures (polymethylmethacrylate), catheters (silicone elastomer), and contact lenses (soft contact lenses).
Bacterial and mixed species biofilms are also a specialty of the laboratory. Quantification of biofilms is performed using colorimetric assays that determine metabolic activity and dry weight determination, which measures the total biomass of biofilms. Morphology and architecture of biofilms are visualized using fluorescence, SEM, and confocal scanning laser microscopy microscopic techniques. Assessment of the activity of antifungal and/or antibacterial agents against biofilms is assessed using the XTT-based metabolic activity assay.
NTS has access to an established ~50,000 sample library of relevant microbial isolates and defined microbial populations (“defined flora”).
NTS has access to an established ~50,000 sample library of relevant microbial isolates and defined microbial populations (“defined flora”), which currently exists in the University Hospitals Cleveland Medical Center and the Center for Medical Mycology and under the direction of Dr. Ghannoum. This repository can facilitate standardized cultivation of microbial or fungal organisms based on customer requests. One highlighted capability is the standardized platforms available for the growth and expansion of microbial, fungal, and mixed species biofilms.
Biofilm Formation by the Fungal Pathogen Candida Albicans: Development, Architecture, and Drug Resistance
Biofilms are a protected niche for microorganisms, where they are safe from antibiotic treatment and can create a source of
Mechanism of Fluconazole Resistance in Candida Albicans Biofilms: Phase-specific Role of Efflux Pumps and Membrane Sterols
Candida albicans biofilms are formed through three distinct developmental phases and are associated with high fluconazole (FLU) resistance. In the
Interaction of Candida Albicans with Adherent Human Peripheral Blood Mononuclear Cells Increases C. Albicans Biofilm Formation and Results in Differential Expression of Pro- and Anti-inflammatory Cytokines
Monocytes and macrophages are the cell types most commonly associated with the innate immune response against Candida albicans infection. Interactions
Fusarium and Candida Albicans Biofilms on Soft Contact Lenses: Model Development, Influence of Lens Type, and Susceptibility to Lens Care Solutions
Fungal keratitis is commonly caused by Fusarium species and less commonly by Candida species. Recent outbreaks of Fusarium keratitis were
In Vitro Growth and Analysis of Candida Biofilms
Evaluation of fungal biofilm formation can be performed using several techniques. In this protocol, we describe methods used to form